Required knowledge
A good knowledge of the following methods, terms, principles and the context in which they are relevant is a precondition for Medical Life Sciences. The topics listed below contain the MINIMUM knowledge requirements.
Lab experience is a must, i.e. you have conducted lab experiments yourself; having watched how steps were demonstrated does not count.
Cell biology knowledge
eukaryotes/prokaryotes
DNA replication
transcription
translation
molecular cloning
cell structure and organelles
Western/Southern blot
cell cultures
ELISA
Relevant literature: Alberts, Bruce, Molecular Biology of the Cell (6th edition, 2014) - [Part I: introduction to the cell, II: basic genetic mechanisms, III: manipulating proteins, DNA, RNA]
Genetics and molecular biology knowledge
PCR
molecular cloning
Sanger sequencing
nucleic acid hybridisation
human chromosomes, Mendelian inheritance
gene structure
Relevant literature: Strachan, Thomas and Read, Andrew, Human Molecular Genetics (5th edition 2018)
Biochemistry knowledge
Carbohydrates
Proteins
Lipids
Relevant literature: Berg JM, Gumport RI, Stryer L et al., Biochemistry (9th edition 2019)
Major principles in biology and scientists establishing them:
Theory of Evolution
Natural Selection
Charles Darwin
Jean-Baptiste de Lamarck
Minimum lab skills
Calculating molarities
DNA extraction
PCR
Gel electrophoresis
Gel preparation
Pipetting with microlitre pipettes (e.g. Eppendorf pipettes)
Buffer preparation
Handling enzymes
The importance of handling yourself in a laboratory
It is very helpful to have done additional lab internships of several weeks or months in the industry or in academia carrying out tasks in the lab self-guidedly. The minimum requirement is lab experience in your Bachelor studies of several weeks (genetics, cell biology, biochemistry) and with the lab skills cited above in place.
